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为了研究新麦草属种间的遗传多样性和寻找新麦草属 N 染色体组在小麦背景下的生化标记,利用等电聚焦电泳对小麦品种中国春(对照种)、P.fragilis、单花新麦草、华山新麦草、新麦草4个种进行了6个生化位点分析。此外,利用聚丙烯酰胺凝胶电泳对1份新麦草进行了醇溶蛋白分析。结果表明:1.异花授粉的新麦草属植物,在所分析的生化位点上,不仅种间而且种内材料间均存在广泛的多态性;2.尽管新麦草属植物存在遗传上的异质性,但在 Est-5中可找到该属 N 基因组的标志带;3.Est-5、Est-6、Ibf-1位点上的特征带,既可作为鉴别新麦草属4个种的种间生化标记,也可作为在中国春遗传背景下鉴定这4个种中携带这些位点的染色体的生化标记。研究还发现,新麦草的 Gli-1和 Est-5两个生化标记带谱丰富,多态性强,是进行新麦草属种质资源指纹图谱绘制及遗传多样性研究较好的生化标记。
In order to study the genetic diversity of the genus Pulsatilla and to search for the biochemical markers of the genus Nectarine in the wheat background, isoelectric focusing electrophoresis was applied to analyze the genetic diversity of Chinese wheat (P.cultured), P.fragilis, , Huashan new wheat grass, wheat straw four biochemical sites were analyzed. In addition, 1 part of wheatgrass was analyzed for gliadin by polyacrylamide gel electrophoresis. The results showed as follows: 1. Alloplasmic pollination of Pulsatilla sp., In the analyzed biochemical sites, not only species but also a wide range of polymorphism between the species of the material; 2. Although new wheat genus genetic Heterogeneity, but in the Est-5 can be found in the N genome marker band; 3.Est-5, Est-6, Ibf-1 locus characteristic bands, both as a new genus to identify four species Of the interspecific biochemical markers, but also can be used as a Chinese spring genetic background identification of these four species carry these loci chromosome biochemical markers. The study also found that two biomarkers of Gli-1 and Est-5 in P. annua were rich in biochemical markers and strong in polymorphism, which was a biochemical marker for mapping and genetic diversity of New Wheatgrass germplasm resources.