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目的:评价紫杉醇对放射抗拒鼻咽癌细胞的放射增敏及其机制的初步研究。方法:通过X射线大剂量低分割照射技术建立鼻咽癌放射抗拒细胞亚系(子代,CNE-2S1);用紫杉醇单独处理和联合放疗分别处理CNE-2S1,以单纯放疗处理组为对照组。通过细胞克隆形成实验观察紫杉醇对放疗的增敏作用;采用流式细胞仪分析不同处理组CNE-2S1的细胞周期分布及凋亡发生率;免疫印迹分析不同处理组SHP-1表达水平。结果:成功建立鼻咽癌放射抗拒细胞亚系CNE-2S1;细胞克隆实验结果显示紫杉醇对放疗具有明显增敏作用;流式检查结果表明紫杉醇处理后的CNE-2S1处于G2-M期细胞比例明显增高,紫杉醇联合放疗处理组可明显提高CNE-2S1细胞的凋亡比率;免疫印迹结果显示紫杉醇及联合放疗组可降低CNE-2S1细胞中SHP-1的表达水平。结论:紫杉醇可增强放疗对鼻咽癌放射抗拒细胞的作用,SHP-1下调可能参与其中。
OBJECTIVE: To evaluate the radiosensitization of paclitaxel on radioresistant nasopharyngeal carcinoma cells and its mechanism. Methods: The nasopharyngeal carcinoma radioresistant cell subline (CNE-2S1) was established by high-dose and low-dose X-ray irradiation. CNE-2S1 was treated with paclitaxel alone and in combination with radiotherapy. . The effect of paclitaxel on radiosensitization was observed by cell clone formation assay. The cell cycle distribution and apoptosis rate of CNE-2S1 in different treatment groups were analyzed by flow cytometry. The SHP-1 expression levels in different treatment groups were analyzed by immunoblotting. Results: The nasopharyngeal carcinoma radiation-resistant cell line CNE-2S1 was successfully established. The results of cell cloning experiments showed that paclitaxel had a significant sensitizing effect on radiotherapy. The results of flow cytometry showed that the percentage of cells in G2-M phase of CNE-2S1 treated with paclitaxel was significantly higher Increased paclitaxel combined with radiotherapy could significantly increase the apoptosis rate of CNE-2S1 cells. Immunoblotting showed that paclitaxel combined with radiotherapy could reduce the expression of SHP-1 in CNE-2S1 cells. Conclusion: Paclitaxel can enhance radiotherapy of nasopharyngeal carcinoma radiosensitive cells, SHP-1 down may be involved.