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目的:探讨子宫颈鳞状细胞癌组织中血管生成拟态(vasculogenic mimicry,VM)和EphA2(ervthropoietin producing hepatocelluar carcinoma A2)的表达及意义。方法:收集宫颈鳞状细胞癌石蜡标本60例及慢性宫颈炎石蜡标本20例。应用PAS及CD34免疫组化双重染色检测宫颈鳞癌组织中是否存在VM;采用免疫组织化学方法检测EphA2蛋白在宫颈鳞癌组织中的表达特点。结果:宫颈鳞癌组织中VM表达阳性率为40.0%(24/60),显著高于慢性宫颈炎组织(P<0.05);VM在中低分化组的阳性率(47.9%)高于高分化组(8.3%),与FIGO分期、淋巴结转移有关(P<0.05);慢性宫颈炎和宫颈癌组织中E-phA2蛋白阳性表达的差异有统计学意义(P<0.05);EphA2的表达与病理分级及淋巴结转移有关(P<0.05);VM与EphA2的表达呈正相关(r=0.362,P<0.05)。结论:宫颈鳞癌组织中存在VM,EphA2高表达可能促进VM;VM与EphA2高表达与宫颈癌的侵袭、转移密切相关;VM与EphA2联合检测对宫颈癌的进展及预后判断有重要意义。
Objective: To investigate the expression and significance of vasculogenic mimicry (VM) and EphA2 (hepatocellular carcinoma A2) in cervical squamous cell carcinoma. Methods: Sixty cases of cervical squamous cell carcinoma paraffin specimens and 20 cases of chronic cervicitis paraffin specimens were collected. The expression of EphA2 protein in cervical squamous cell carcinoma was detected by immunohistochemical method using PAS and CD34 double staining to detect the existence of VM in cervical squamous cell carcinoma. Results: The positive rate of VM in cervical squamous cell carcinoma was 40.0% (24/60), significantly higher than that in chronic cervicitis (P <0.05). The positive rate of VM in moderate and poorly differentiated group (47.9%) was higher than that in chronic cervicitis (P <0.05). The difference of E-phA2 protein expression in chronic cervicitis and cervical cancer was statistically significant (P <0.05). The expression of EphA2 and pathology Grade and lymph node metastasis (P <0.05). There was a positive correlation between VM and EphA2 expression (r = 0.362, P <0.05). Conclusion: The high expression of VM and EphA2 in cervical squamous cell carcinoma may promote the VM. The high expression of VM and EphA2 is closely related to the invasion and metastasis of cervical carcinoma. The combined detection of VM and EphA2 is of great significance for the progression and prognosis of cervical carcinoma.