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本文报告了短膜虫(Crithidia fasculata)酪氨酸转氨酶(TAT)的纯化和鉴定方法。TAT在辅酶存在时,催化酪氨酸与适当酮酸间的氨基转移作用。用改良Diamondstone法检测其活性,每份分析液总量为0.93ml,其中含4mM L-酪氨酸、50mM磷酸吡哆醛、10mM α-酮戊二酸和0.1ml酶样品。以上各成分均以100mM pH7.6磷酸钾缓冲液处理。37℃反应15分钟。以0.07ml 10N KOH
This article reports on the purification and characterization of Crithidia fasculata tyrosine aminotransferase (TAT). TAT catalyzes the transamination of tyrosine with the appropriate keto acid in the presence of the coenzyme. Their activity was tested by the modified Diamondstone method. The total amount of each assay was 0.93 ml containing 4 mM L-tyrosine, 50 mM pyridoxal phosphate, 10 mM α-ketoglutarate and 0.1 ml enzyme sample. The above ingredients were all treated with 100 mM pH 7.6 potassium phosphate buffer. 37 ° C for 15 minutes. 0.07 ml 10 N KOH