论文部分内容阅读
该文从杨树 -光肩星天牛的协同进化关系出发 ,应用同工酶分析法对毛白杨、I 69杨和小美旱杨进行了生态抗虫性的研究 .根据聚丙烯酰胺凝胶电泳方法对对照树的酶谱分析表明 ,3种杨树的过氧化物酶 (POD)、多酚氧化酶 (PPO)和酯酶 (EST)各有其特有的酶谱 ,毛白杨POD具有特异性条带 ;根据比色方法对酶活性分析得毛白杨POD含量最高 ,活性最强 ,通过方差分析和多重比较得 ,毛白杨与其它两种杨树之间的POD酶差异极显著 .随后又对危害树进行了分析 ,测得毛白杨POD增长率最大 (为 17% ) .由此作者认为POD ,PPO ,EST和蛋白质 (PRO)都与抗虫性有一定的关系 ,其中POD与抗虫性的关系最大 ,可能由抗虫基因所控制 .
Based on the symbiosis between Populus euphratica and Anoplophora glabripennis, this paper studied the ecological insect resistance of Populus tomentosa, Populus tomentosa and Populus davidiana by isozyme analysis.According to polyacrylamide gel electrophoresis The zymogram analysis of the tree showed that the peroxidase (POD), polyphenol oxidase (PPO) and esterase (EST) of the three poplar species had their own unique enzyme patterns, POD of Populus tomentosa had specific bands; According to the colorimetric method, the POD content of Populus tomentosa was the highest and the activity was the strongest, the difference of POD enzyme between Populus tomentosa and the other two poplars was extremely significant by analysis of variance and multiple comparisons.And then, (POD, PPO, EST and protein (PRO)) had a certain relationship with insect resistance, of which the relationship between POD and insect resistance was the highest Controlled by insect-resistant genes.