论文部分内容阅读
目的:优化α-细辛脑脂质体的制备工艺。方法:以薄膜分散法制备α-细辛脑脂质体,Box-Behnken设计响应面法优化α-细辛脑脂质体的制备工艺。采用包封率(Y)及稳定性参数(Ke)为优化指标,考察磷脂浓度(X1)、磷脂/胆固醇(X2)、磷脂/药物(X3)对制备工艺的影响。结果:建立了二项式拟合方程,方程拟合度高,最佳工艺条件为:磷脂浓度3.99%、磷脂与胆固醇的重量比4.94、磷脂与药物的重量比39.21。在此工艺条件下,脂质体的包封率为48.65%,稳定性常数Ke为3.80。结论:以包封率及稳定性常数Ke为指标,Box-Behnken设计有效地优化α-细辛脑脂质体的制备工艺,所得工艺稳定可靠,为α-细辛脑新剂型研究提供参考。
Objective: To optimize the preparation of α-asarone liposomes. Methods: The α-Aspergillus liposomes were prepared by the thin-film dispersion method. The preparation of liposomes was optimized by Box-Behnken design. The effects of phospholipid concentration (X1), phospholipid / cholesterol (X2) and phospholipid / drug (X3) on the preparation process were investigated using entrapment efficiency (Y) and stability parameter (Ke) as optimization parameters. Results: The fitting equation of binomial equation was established. The fitting equation of the equation was high. The optimum technological conditions were as follows: phospholipid concentration 3.99%, phospholipid - cholesterol weight ratio 4.94 and phospholipid - drug weight ratio 39.21. Under these conditions, the entrapment efficiency of liposomes was 48.65%, and the stability constant Ke was 3.80. Conclusion: The entrapment efficiency and stability constant Ke as an index, Box-Behnken design effectively optimize the preparation process of α-Aspergillum liposomes, the resulting process is stable and reliable, a-Asarone new formulations for reference.