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蔬莱中埃希氏大肠杆菌的酶-底物快速检测法，以FCM培养液中添加MUG作为与埃希氏菌在培养过程中生成的β-葡萄糖苷酸酶反应的底物，埃希氏菌则作为蔬菜提取物样品加入培养液中．阳性结果系根据MUG被酶作用后的水解产物在3650A紫外线照射下所产生的蓝色荧光反应．培养物中MUG的合适剂量为50μg／mL，获得阳性结果时间不超过24h，本法与传统的多管发酵法比较结果表明，在检测粪源性污染的蔬菜样品时，它的敏感性及准确性与多管发酵法相一致。
Rapid detection of Escherichia coli in Escherichia coli by enzyme-substrate assay, MUG addition in FCM broth as substrate for β-glucuronidase reaction with Escherichia coli during culture, The bacteria are then added to the culture broth as a vegetable extract sample. The positive result is based on the blue fluorescence reaction of the MUG enzyme hydrolyzate under 3650A UV irradiation. The suitable dose of MUG in culture is 50μg / mL, and the positive result does not exceed 24 hours. Compared with the traditional multi-tube fermentation method, this method shows that it is sensitive and accurate in detecting fecal contamination of vegetable samples Sex and multi-tube fermentation method is consistent.