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[目的]探讨缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)及基质金属蛋白酶-9(MMP-9)在非小细胞肺癌(NSCLC)组织中的表达.[方法]采用免疫组化法检测91例NSCLC组织及相对应的癌旁正常组织中hIF-1α、VEGF及MMP-9的阳性表达,并分析其与NSCLC临床病理参数的关系.[结果]NSCLC中HIF-1α、VEGF及MMP-9阳性率均显著高于癌旁正常组织(P<0.05);低分化NSCLC中hIF-1α、VEGF及MMP-9阳性率高于中高分化(P<0.05);Ⅲ~Ⅳ期的NSCLC中hIF-1α、VEGF及MMP-9阳性率高于Ⅰ~Ⅱ期(P<0.05),有淋巴结转移的NSCLC中hIF-1α、VEGF及MMP-9阳性率高于无淋巴结转移的(P0.05);NSCLC中hIF-1α、VEGF及MMP-9阳性表达呈两两正相关(P<0.05).[结论]HIF-1α、VEGF及MMP-9在NSCLC组织中高表达,且与病情的发生发展相关,三者联合检测可能为NSCLC的预后评估及靶向治疗提供依据.“,”[Objective]To investigate the expression of hypoxia-inducible factor-1α(HIF-1α),vascular en-dothelial growth factor(VEGF)and matrix metalloproteinase-9(MMP-9)in non-small cell lung cancer (NSCLC)and explore their clinical applications.[Methods]The expression of HIF-1α,VEGF and MMP-9 was measured by immunohistochemistry in 91 cases of NSCLC and corresponding adjacent normal tissues.The re-lationship between these three factors and the clinicopathological parameters of NSCLC was analyzed.[Re-sults] The expression of HIF-1α,VEGF and MMP-9 in cancer tissue of NSCLC was higher than that in adja-cent normal tissues(P<0.05).The positive rates of HIF-1α,VEGF and MMP-9 in low differentiated NSCLC were higher than those in moderate and highly differentiated NSCLC(P<0.05).The positive rates of HIF-1α,VEGF and MMP-9 in Ⅲ ~ Ⅳ stage were higher than those in Ⅰ ~ Ⅱ stage(P<0.05).The positive rates of HIF-1α,VEGF and MMP-9 in NSCLC with lymph node metastasis were higher than those in non-lymph node metastasis(P<0.05).The expression of HIF-1α,VEGF and MMP-9 in NSCLC tissue was not correlated with age,sex,tumor diameter and pathological type.The positive expression of HIF-1α,VEGF and MMP-9 in NSCLC was positively related with one another(P<0.05).[Conclusion]HIF-1α,VEGF and MMP-9 promoted the occurrence and development of NSCLC;the combined detection of these three factors may provide a more valuable prognosis evaluation and target therapy of NSCLC.