论文部分内容阅读
利用Ｔ－载体克隆法完成了含信号肽及前导肽的人脑源性神经营养因子（ｂｒａｉｎ－ｄｅｒｉｖｅｄｎｅｕｒｏｔｒｏｐｈｉｃｆａｃｔｏｒ，ＢＤＮＦ）全长基因ＰＣＲ产物的克隆。序列测定结果表明，所克隆的人ＢＤＮＦ基因从起始密码子ＡＴＧ到终止密码子ＴＡＧ的７７４ｂｐ中，除下游端ＰＣＲ引物因为采用猪的ＰＣＲ引物而有一个碱基改变外，其它序列与国外文献报道序列完全一致。该碱基改变不影响氨基酸序列
The cloning of full-length PCR product of brain-derived neurotrophic factor (BDNF) containing signal peptide and leader peptide was completed by T-vector cloning method. Sequence analysis showed that the cloned human BDNF gene from the start codon ATG to the stop codon TAG 774bp, except for the downstream PCR primers because of the use of porcine PCR primers and a base change, the other sequences and foreign literature Report sequence exactly the same. This base change does not affect the amino acid sequence