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目的:探究SQSTM1在甲状腺乳头状癌中的表达及其对甲状腺乳头状癌细胞TPC-1侵袭和迁移能力的影响。方法:采集2019年4至6月郑州大学第一附属医院收治的21例甲状腺乳头状癌患者切除手术的癌组织和癌旁组织，通过逆转录-聚合酶链反应（RT-qPCR）方法检测组织中SQSTM1的表达情况；通过慢病毒转染在TPC-1细胞中构建SQSTM1缺失细胞系SQSTM1-KD-TPC-1， RT-qPCR从基因水平检测SQSTM1在TPC-1 和SQSTM1-KD-TPC-1细胞中的表达；transwell法检测SQSTM1敲低前后TPC-1侵袭和迁移的变化情况；MTT和平板克隆形成实验检测SQSTM1敲低后TPC-1细胞增殖能力的变化；通过RT-qPCR检测增殖相关蛋白的表达情况，从基因水平进一步验证肿瘤细胞对增殖能力的影响。结果:甲状腺乳头状癌组织中SQSTM1的表达明显高于正常癌旁组织，有76.2%（16/21）的患者mRNA呈现高表达；敲低SQSTM1明显抑制肿瘤增殖、侵袭和迁移的能力，且增殖相关蛋白表达明显减小（n P均<0.01），表明SQSTM1参与增殖相关通路机制的调控。n 结论:SQSTM1在甲状腺乳头状癌细胞株TPC-1中明显促进细胞侵袭、迁移和增殖，可能是潜在的基因治疗靶点。“,”Objective:To investigate the expression of SQSTM1 in thyroid papillary carcinoma and its influence on the invasion and migration of thyroid papillary carcinoma cells TPC-1.Methods:From April to June 2019, cancer tissues and adjacent tissues of 21 cases with thyroid papillary carcinoma in the First Affiliated Hospital of Zhengzhou University were collected, and the expression of SQSTM1 was detected by RT-qPCR. SQSTM1 knockdown cell line SQSTM1-KD-TPC-1 was constructed in TPC-1 cells by lentivirus transfection. RT-qPCR was used to detect SQSTM1 expression in TPC-1 cells and SQSTM1-KD-TPC-1 cells. The changes of invasion and migration before and after SQSTM1 knockdown in TPC-1 cells were detected by transwell test. The proliferation of TPC-1 and SQSTM1-KD-TPC-1 cells were detected by MTT and clone formation test. RT-qPCR was used to detect the gene expression of proliferation related proteins.Results:The expression of SQSTM1 in papillary thyroid carcinoma tissues was significantly higher than that in normal adjacent tissues, and 76.2%(16/21) of the petients showed high mRNA expression. Knock down SQSTM1 significantly inhibited the ability of tumor proliferation, invasion and migration, and the expression of proliferation-related proteins were significantly decreased (n P<0.01), indicating that SQSTM1 was involved in the regulation of proliferation related pathway mechanism.n Conclusion:SQSTM1 significantly promotes invasion, migration and proliferation in thyroid papillary cancer cells TPC-1 and may be a potential gene therapy target.