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目的 检测中国人胃癌组织及相应癌旁组织中的端粒酶活性，探讨将其作为胃癌肿瘤标志物的可能性。方法 采用以ＰＣＲ为基础的ＴＲＡＰ（ｔｅｌｏｍ ｅｒｅ ｒｅｐｅａｔａｍ ｐｌｉｆｉｃａｔｉｏｎ ｐｒｏｔｏｃｏｌ）方法检测了４２ 例胃癌组织、４２ 例相应癌旁组织及１ 例胃溃疡伴肠上皮化生及轻度不典型增生组织中的端粒酶活性表达。结果 ４２ 例胃癌组织中，３７ 例显示端粒酶活性表达阳性，阳性率为８８１％ ；而在４２ 例相应癌旁组织中，仅有２ 例显示端粒酶活性表达阳性，１ 例胃溃疡伴肠上皮化生及轻度不典型增生组织端粒酶活性表达阳性。结论 以上结果提示端粒酶检测有可能成为胃癌辅助性诊断手段
Objective To detect the telomerase activity in gastric cancer tissues and corresponding paracancerous tissues in Chinese and to explore its possibility as a tumor marker for gastric cancer. Methods PCR-based TRAP (telom ere repeatamplification protocol) was used to detect telomeres in 42 cases of gastric cancer, 42 cases of corresponding para-carcinoma tissues, and 1 case of gastric ulcer with intestinal metaplasia and mild dysplasia. Enzyme activity expression. Results In 42 cases of gastric cancer, 37 cases showed positive expression of telomerase activity with a positive rate of 88.1%. In 42 cases of corresponding adjacent tissues, only 2 cases showed positive expression of telomerase activity and 1 case of stomach. The expression of telomerase activity was positive in ulceration with intestinal metaplasia and mild atypical hyperplasia. Conclusion The above results suggest that telomerase detection may become a diagnostic aid for gastric cancer