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通过对多茎尖繁殖方法和转基因研究，建立了香蕉茎尖外源基因的遗传转化方法。明确了卡那霉素或Ｇ－４１８在转化植株中所使用的合适浓度，即当使用卡那霉素时，其产梢的浓度为 １００ ｍｇ／Ｌ，导根的浓度为 １５０ ｍｇ／Ｌ；但当使用 Ｇ－４１８时，其产梢浓度则为 ２５ ｍｇ／Ｌ，其导根浓度则为 ３０ ｍｇ／Ｌ或３５ ｍｇ／Ｌ。通过基因枪和农杆菌共培养法尝试将黄瓜花叶病毒的衣壳蛋白基因转化香蕉组织，其结果表明：经过卡那霉素或Ｇ－４１８的筛选，仅有 ５％－７％的芽能够存活下来。通过选择性继代培养，最后获得了７３株转化植株；对其中的２４株进行ＰＣＲ检测，结果表明阳性率达 ８３．３％；进一步的 Ｓｏｕｔｈｅｒｎ ｂｌｏｔ检测表明：ＰＣＲ检测阳性的８１．８％植株含有外源衣壳蛋白基因。
The method of genetic transformation of exogenous genes in banana shoots was established by studying the multiplication and transgenic studies of shoot tips. The appropriate concentration of kanamycin or G-418 to be used in the transformed plants was determined, that is, when kanamycin was used, the shoot tip concentration was 100 mg / L and the rooting concentration was 150 mg / L; However, when G-418 was used, the shoot tip concentration was 25 mg / L and the rooting concentration was 30 mg / L or 35 mg / L. Attempts to transform the capsid protein gene of cucumber mosaic virus into banana tissue by gene gun and Agrobacterium co-culture showed that only 5% -7% of buds could be selected by kanamycin or G-418 screening Survive. By selective subculture, 73 transformed plants were finally obtained, of which 24 strains were tested by PCR. The results showed that the positive rate reached 83.3%. Further Southern blot showed that 81.8% of the plants tested positive by PCR Contains exogenous capsid protein gene.