论文部分内容阅读
目的明确现场种群德国小蠊对溴氰菊酯抗性状态,研究其滞留喷洒的杀蟑效果,以及耐受个体kdr抗性基因型。方法根据相关国标用药膜法检测试虫对溴氰菊酯抗性,用强迫接触法测定在玻璃、清漆木板及水泥3种代表性表面滞留喷洒药效,用钠离子通道基因DNA扩增产物测序确定基因型。结果随着表面吸水性增强,对试虫击倒速度降低,持效期缩短;不同种群的抗性状态影响药效,3种表面对敏感及低抗(2.05倍)种群均有较好效果,对中抗(5.17倍)种群效果较差。对杀虫剂耐受的个体均携带kdr抗性基因型,中抗种群中抗性纯合(RR)基因型频率较高,而低抗种群中抗性杂合(RS)基因型频率较高。结论滞留喷洒防治中,德国小蠊对杀虫剂抗性表型与其携带的抗性基因型密切相关,不恰当的防治反而增加种群中抗性基因频率,因此要根据环境类型、靶标抗性状态与用药史,调整施药次数与剂量,并将滞留喷洒与胶、毒饵等手段结合,从而达到理想的防治效果。
Objective To determine the resistance status of Blattella germanica to deltamethrin in the field and study its killing effect by stranded spray and its kdr resistant genotype. Methods The resistance of test insects to deltamethrin was tested according to the relevant national standard membrane method. The force-contact method was used to determine the spraying efficacy on the three representative surfaces of glass, varnish wood and cement. The DNA amplification products of sodium ion channels were sequenced Determine genotype. Results As the surface water absorption increased, the knock-down speed of pests decreased and the holding period shortened. The resistance status of different populations affected the efficacy. The three kinds of surfaces had good effect on the sensitive and low-resistant (2.05-fold) populations, The resistance (5.17 times) population less effective. The kdr resistant genotypes were all carried in the insecticide resistant individuals, but the frequency of the resistant homozygous (RR) genotypes in the resistant population was higher than that in the resistant population . Conclusions In the control of retention spraying, the resistant phenotype of Blattella germanica to insecticides is closely related to the resistant genotypes it carries. Improper control may increase the frequency of resistance genes in the population, and therefore, according to the type of environment, the target resistance status And medication history, adjust the number of doses and doses, and the retention spray and glue, bait and other means combined to achieve the desired effect of prevention and treatment.