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Recently,many resistance genes have beencloned from crop species.The structures ofproducts of these genes are similar,such asnucleotide binding sites(NBS),leucine-richrepeat(LRR)motif,and serine-threonine ki-nase domain(STK). Our experiments were designed to detectthe expression proportion that were altered inabundance with filtrated bacteria culture solu-tion in rice lines IR26(resistant to XOO)andJingang 30(susceptible).The times of ricecallus exposed in culture solution were 0,5,30,and 60 min and the expression of mRNAwas analyzed using combinations of five degen-erate DD-PCR primers and three T12M
Recently, many resistance genes have been cloned from crop species. These structures of products of these genes are similar, such as nucleotide binding sites (NBS), leucine-richrepeat (LRR) motif, and serine-threonine ki-nase domain (STK) were designed to detect the expression proportion that were altered in abundance with filtrated bacteria culture solu- tion in rice lines IR26 (resistant to XOO) and Jingang 30 (susceptible). times times rice callus exposed in culture solution were 0,5,30, and 60 min and the expression of mRNA were analyzed using combinations of five degen-erate DD-PCR primers and three T12M