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应用中国预防医学科学院病毒学研究所提供的庚型肝炎病毒(HGV)RT-nested PCR 检测试剂,对我院1995年1月～8月住院患者中41例 EBV、CMV 感染阴性的 non A～E肝炎患者血清进行了 HGV RNA 检测,结果如下。一、流行病学资料对41例 non A～E肝炎患者进行了 HGV RNA 检测,39例急性肝炎6例阳性,2例慢性肝炎1例阳性,总阳性率为17.07%。7例庚型肝炎患者中男4例、女3例。年龄29岁～62岁,平均年龄为42.57±11.79岁。1例有手术输血史,其余6例均无手术和输血及血制品史,传播途径不清。应用 PCR 方法检测7例 HGV感染患者的血清 HBV DNA 和 HCV RNA,结果均为阴性。二、7例庚型肝炎临床结果分析1.急性庚型肝炎 6例患者均有乏力、纳差、厌油、尿黄,5例有恶心,3例有发热,呕吐、肝区不适各2例。ALT 及总胆
Application of hepatitis G virus (HGV) RT-nested PCR detection reagent provided by the Institute of Virology, Chinese Academy of Preventive Medicine, 41 cases of non-EBV, CMV-negative non A to E in our hospital from January to August 1995 HGV RNA was detected in the serum of hepatitis patients and the results were as follows. First, the epidemiological data of 41 patients with non A to hepatitis E HGV RNA detection, 39 cases of acute hepatitis in 6 cases, 2 cases of chronic hepatitis in 1 case, the total positive rate was 17.07%. Seven cases of hepatitis G patients, 4 males and 3 females. Aged 29 to 62 years old, with an average age of 42.57 ± 11.79 years. One case had the history of operation blood transfusion and the other 6 cases had no history of surgery and blood transfusion and blood products, and the route of transmission was unclear. Serum HBV DNA and HCV RNA in seven HGV-infected patients were detected by PCR, and the results were negative. Second, seven cases of hepatitis G clinical analysis 1. Acute hepatitis G 6 patients were fatigue, anorexia, tired of oil, urine yellow, nausea in 5 cases, 3 cases of fever, vomiting, liver discomfort in 2 cases . ALT and total gall