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目的:研究紫杉醇对人口腔鳞癌KB细胞生长抑制及凋亡的作用。方法:MTT方法检测紫杉醇对人口腔鳞癌KB细胞生长抑制率,计算IC50;将0.25,0.5,1μmol·L-1的紫杉醇作用于KB细胞48 h,分别用DAPI染色和流式细胞术检测细胞凋亡,并用免疫印迹法检测紫杉醇对凋亡相关蛋白Bax,Bcl-2和pro-caspase-3的影响。结果:紫杉醇对KB细胞的生长有明显的抑制作用,DAPI染色可见典型的凋亡细胞形态学特征,流式细胞术检测结果表明紫杉醇增加KB细胞凋亡率,免疫印迹结果显示紫杉醇可以增加Bax表达,减少Bcl-2表达,激活caspase-3。结论:紫杉醇对人口腔鳞癌KB细胞有生长抑制和诱导凋亡作用,这可能与抗癌机制有关。
Objective: To study the effect of paclitaxel on growth inhibition and apoptosis of human oral squamous cell carcinoma KB cells. Methods: MTT assay was used to detect the inhibitory rate of paclitaxel on human oral squamous cell carcinoma KB cells. IC50 was calculated. Taxol, 0.25, 0.5 and 1 μmol·L-1 were treated with KB cells for 48 h. The cells were detected by DAPI staining and flow cytometry Apoptosis was detected by immunohistochemistry. The effects of paclitaxel on apoptosis-related proteins Bax, Bcl-2 and pro-caspase-3 were detected by Western blotting. Results: Paclitaxel significantly inhibited the growth of KB cells. The typical morphological features of apoptotic cells were observed by DAPI staining. The results of flow cytometry showed that paclitaxel increased the apoptotic rate of KB cells. Western blotting showed that paclitaxel could increase the expression of Bax , Reduce Bcl-2 expression, activate caspase-3. Conclusion: Paclitaxel can inhibit the growth of human oral squamous cell carcinoma KB cells and induce apoptosis, which may be related to the anti-cancer mechanism.