论文部分内容阅读
以1,3-二苯基异苯并呋喃(1,3-Diphenylisobenzofuran,DPBF)为荧光探针,研究了姜黄素(Curcumin,CUR)在铜离子催化下产生的单线态氧(1O2),其反应机理为姜黄素与溶液中的氧分子快速作用产生O2.-和H2O2等活性氧物种,同时Cu2+与姜黄素形成复合物,再与H2O2形成过氧化物过渡态,过氧化物进一步与H2O2发生类Haber-Weiss反应生成1O2,且只有Cu2+和Cu+离子可催化姜黄素并产生1O2.1O2在1.37×10-8~3.66×10-7mol/L浓度范围内与荧光强度下降值ΔIF有良好的线性关系,检出限为4.12×10-9mol/L.
Using 1,3-diphenylisobenzofuran (DPBF) as a fluorescent probe, the singlet oxygen (1O2) produced by curcumin (CUR) catalyzed by copper ions was studied. The reaction mechanism is that curcumin rapidly interacts with oxygen molecules in the solution to generate reactive oxygen species such as O2.- and H2O2, and at the same time, Cu2 + forms a complex with curcumin, forms a peroxide transition state with H2O2, and peroxide further interacts with H2O2 Class Haber-Weiss reaction produces 1O2, and only Cu2 + and Cu + ions can catalyze the curcumin and produce 1O2.1O2 in the concentration range of 1.37 × 10-8 ~ 3.66 × 10-7mol / L and the fluorescence intensity decrease ΔIF has a good linear The detection limit was 4.12 × 10-9mol / L.