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目的 从宿主动物及蜱中分离斑点热群立克次体 ,以了解广东省是否存在斑点热自然疫源地。方法 从广东省封开县七星自然保护区采用笼日法捕活鼠 ,采集鼠脾及鼠表的蜱 ,用PCR进行初筛 ,鸡胚卵黄囊培养法直接分离立克次体 ,血清学试验对分离株进行鉴定。结果 采集鼠脾10 3份 ,鼠表的蜱 38匹 ;从 2只针毛鼠体表捉获的蜱中分离到 2株斑点热群立克次体 ,命名为GDFK5 8- 2 0 0 0株、GDFK5 9- 2 0 0 0株立克次体 ,经血清学鉴定为西伯利亚种。结论 首次从病原学上证实了广东省存在斑点热自然疫源地
OBJECTIVES: To isolate speckle fever group Rickettsia from host animals and ticks in order to find out whether there is a spotted hot natural foci in Guangdong Province. Methods The flies were collected from the spleen and mouse by using the cage method in Qixing Nature Reserve of Fengkai County, Guangdong Province. The ticks were collected by PCR. The chicken embryo yolk sac culture method was used to separate rickettsia. Serological tests Isolates were identified. Results Totally 103 spleens of mouse spleen and 38 ticks of mouse were collected. Two speckles of Rickettsia glabra were isolated from the ticks captured on the body surface of two needle-feeders, named as GDFK5 8- 20 000 , GDFK5 9-2 0 0 0 strains Rickettsia, serologically identified as Siberian species. Conclusions The etiology of plague origin in Guangdong province was confirmed for the first time