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利用生物信息学手段对茶树NAC转录因子家族的成员、系统发育、编码蛋白的理化性质和结构以及创伤胁迫处理后的表达进行分析.研究结果显示,茶树NAC转录因子家族包含49个成员,与拟南芥的106个NAC成员构建系统发育进化树,结果显示,茶树缺乏拟南芥NAC家族17个亚族中的第10和第14两个亚族.理化性质和结构分析显示茶树NAC蛋白质绝大多数是亲水氨基酸,二级结构以无规则卷曲为主,三级结构大部分相似.保守基序分析表明,茶树NAC成员共包含7个保守基序,其中基序3、4、2和5、1分别代表NAC结构域的A、B、C和D亚结构域,而基序7表示的是亚结构域E.第15,16和17亚族的大部分成员都缺失了第2和第4两个保守基序,而第15亚族成员具有一个特异的保守基序6.在机械创伤处理条件下表达模式分析表明,第11亚族的CsNAC33和CsNAC34两个成员虽然具有不一样的蛋白结构域组成,但在应对创伤处理时呈现相对一致的表达模式,而16亚族的CsNAC47和CsNAC48两个成员具有一致的蛋白结构域组成,但其表达模式出现分化.上述结果为后续进行茶树NAC基因家族功能的研究提供了理论依据.
The members of NAC transcription factor family, phylogeny, the physicochemical properties and structure of the encoded protein and the expression after traumatic stress treatment were analyzed by bioinformatics methods.The results showed that the NAC transcription factor family of tea tree contains 49 members, The phylogenetic tree was constructed by 106 NAC members of Arabidopsis thaliana and the results showed that the tea tree lacks the tenth and the fourteenth subfamilies of the 17 subfamilies of the NAC family of Arabidopsis thaliana Physicochemical properties and structural analysis show that the tea tree NAC protein is extremely large Most of them are hydrophilic amino acids, the secondary structure is dominated by random curls, and the tertiary structures are mostly similar.The conserved motif analysis shows that the NAC members of tea tree contain a total of seven conserved motifs, of which motifs 3, 4, 2 and 5 , 1 respectively represent the A, B, C and D subdomains of the NAC domain, while motif 7 represents the subdomain E. Most members of the 15th, 16th, and 17th subfamilies are missing the 2nd and the 4th 4 two conserved motifs, while members of the 15th subfamily have a specific conserved motif.6 Analysis of expression patterns under mechanical wounding conditions shows that although the two members of the eleventh subclass CsNAC33 and CsNAC34 have different proteins Domain composition, but in response However, the 16 subfamilies of CsNAC47 and CsNAC48 had consistent protein domain composition but their expression patterns were differentiated.These results provide the basis for further study on the function of NAC gene family in tea tree Theoretical basis.