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目的 观察死胎脐带组织中HBV -DNA表达的情况 ,探讨通过产妇传播到死胎脐带组织中的HBV是否存在复制。方法 采集 4 0例乙型肝炎产妇产下的死胎 ,常规尸检 ,取脐带组织 ,采用原位分子杂交法 ,检测其中的HBV -DNA ;回访产妇产前静脉血HBV的检测结果。结果 死胎脐带组织中HBV -DNA的检出率为 16 /4 0 (40 % )例。产妇产前静脉血HBV呈高、低和无复制状态时 ,脐带组织中HBV -DNA的检出率分别为 1/ 4 (2 5 % )例、7/ 2 0 (35 % )例和 8/ 16 (5 0 % )例 ;高、低、无复制者之间相互比较 ,差异不显著 (P >0 0 5 )。HBV -DNA颗粒在死胎脐带血管内皮细胞浆、血管中呈点、灶状分布 ,但脐带血管内皮细胞核上无HBV -DNA存在。结论 死胎脐带组织中有HBV复制 ;死胎脐带组织中HBV -DNA表达与产妇血清中HBV复制状态无关。
Objective To observe the expression of HBV-DNA in the fetal umbilical cord tissue and to explore whether there is replication of HBV in maternal umbilical cord tissue. Methods Totally 40 stillbirths, routine autopsy and fetuses were collected from 40 pregnant women with hepatitis B and HBV-DNA was detected by in situ hybridization. The HBV test results were retrospectively analyzed. Results The detection rate of HBV-DNA in stillbirth umbilical cord tissue was 16/40 (40%). The detection rate of HBV-DNA in umbilical cord tissue was 1/4 (25%), 7/20 (35%) and 8 / 16 (50%) cases. There was no significant difference between high, low and no replicas (P> 0.05). HBV-DNA particles in the fetal umbilical cord endothelial cell plasma, blood vessels were spotted, focal distribution, but there is no umbilical cord endothelial cell nucleus HBV-DNA exists. Conclusion There is HBV replication in the fetal umbilical cord tissue. The expression of HBV-DNA in the fetal umbilical cord tissue has nothing to do with the HBV replication in the maternal serum.