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MUG 快速检验法(简称快速法)的检验周期只需29-48小时,而目前药品微生物限度检验中大肠杆菌系按部颁《药品卫生检验方法》进行。该法检验周期长、不能及时报告结果(需4-5天)。MUG 快速法,大大提高了检验效力。MUG 快速法,是将 MUG作为目标菌的基本营养物加入培养基中,被大肠杆菌的β-葡萄糖醛酸酶直接作用,其分解产物又作为一种指示系统,在366nm 紫外光下呈现荧光,即MUG 阳性,若无荧光,则 MUG 阴性。为了比较与部
The test period of the MUG rapid test method (short for the rapid method) is only 29-48 hours, and the current E. coli strains in the microbiological limit test of medicines are carried out according to the Ministry’s “Drug Sanitation Test Method”. The test has a long test cycle and cannot report results in a timely manner (4-5 days). The MUG rapid method greatly improves the test effectiveness. The MUG rapid method uses MUG as the basic nutrient of the target bacteria to be added to the medium and is directly acted on by the β-glucuronidase of E. coli. The decomposition product of the MUG is used as an indicator system to exhibit fluorescence at 366 nm UV light. MUG is positive, if no fluorescence, MUG is negative. For comparison with the Ministry