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目的探讨+Gz重复暴露后大鼠脑组织热休克蛋白70(HSP70)基因表达的变化及其在+Gz所致脑损伤中的作用.方法用半定量反转录聚合酶链反应(RT-PCR)检测方法检测+Gz重复暴露大鼠脑组织HSP70 mRNA表达水平.结果 +Gz重复暴露后30 min、6 h大鼠脑组织HSP70 mRNA均有升高,分别是对照组的1.7倍和2.6倍,24 h恢复正常.结论 +Gz重复暴露可诱导大鼠脑组织HSP70 mRNA的表达, 可能与+Gz所致脑损害后神经细胞的自身保护有关.“,”Objective To study the changes of mRNA expression of heat shock protein 70 (HSP70) in the rat brain exposed to repeated +Gz. Method The mRNA expression levels of HSP70 in rat brain were measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Result The HSP70 mRNA expression levels in rat brains taken 30 min and 6 h after repeated +Gz exposures were significantly higher than those in control group, while the difference between the levels of control group and those of experimental rat brains taken 24 h after +Gz exposure was not significant. Conclusion It is suggested that HSP70 mRNA expression in rat brain can be induced by repeated +Gz exposures and the increased HSP70 mRNA expression may play an important role in self-protection against brain damage induced by+Gz exposures.