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TIFY蛋白是一类植物特有的转录因子,在植物的生长发育、信号传导以及胁迫反应中起重要作用。尽管一些模式植物TIFY家族基因已经被鉴定,但在茄科植物中有关TIFY家族基因的研究却很少。本研究利用生物信息学手段分别从番茄与马铃薯中挖掘到21个Sl TIFY和21个St TIFY基因,并系统性分析这些TIFY基因的序列、表达以及进化特性。染色体定位与扩增模式分析显示,番茄和马铃薯的TIFY基因散落分布在不同染色体上,家族成员扩增主要由串联和片段重复产生。序列分析表明:番茄TIFY基因结构被分为12种,马铃薯TIFY基因结构被分为8种;番茄和马铃薯TIFY蛋白共享了保守基序1,其它保守基序显示不同程度基因特异性,保守基序组成模式表现出高度多样化。系统进化分析显示,番茄和马铃薯TIFY蛋白被分为7大类群,同类群内结构与序列相似性较高。选择压力检测表明,番茄的10对同源基因和马铃薯的19对同源基因均受到负选择作用。表达谱结果表明,番茄和马铃薯TIFY基因的表达呈现较高的多样性,这可能与多样化的功能相关。这些研究结果为功能解析番茄和马铃薯TIFY基因提供理论指导。
TIFY protein is a plant-specific transcription factor that plays an important role in plant growth and development, signaling and stress response. Although some model plant TIFY family genes have been identified, few studies have reported the TIFY family of genes in solanaceous plants. In this study, 21 Sl TIFY and 21 St TIFY genes were excised from tomato and potato using bioinformatics methods, and the sequence, expression and evolution characteristics of these TIFY genes were systematically analyzed. Chromosome mapping and amplification pattern analysis showed that the TIFY genes of tomato and potato were scattered on different chromosomes, and the amplification of family members was mainly generated by tandem and fragment duplication. Sequence analysis showed that the structure of TIFY gene in tomato was divided into 12 species and TIFY gene structure in potato was divided into 8 species. TIFY protein in tomato and potato shared the conserved motif 1, and other conserved motifs showed different degrees of gene specificity. The conserved motif Composition patterns show a high degree of diversity. Phylogenetic analysis showed that TIFY proteins in tomato and potato were divided into seven groups, and the similarities were found in the similar groups. Selection pressure test showed that 10 pairs of homologous genes in tomato and 19 pairs of homologous genes in potato were negatively selected. Expression profiling results showed that TIFY gene expression in tomato and potato showed a high diversity, which may be related to a variety of functions. These findings provide theoretical guidance for the functional analysis of TIFY genes in tomato and potato.