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一、试验材料 1.供试药剂:50％多霉清(保定化工八厂生产),70％甲基托布津(日本曹达株式会社生产)。 2.供试菌种:苹果轮纹病菌(Physalosproa piricola Nose),苹果炭疽病菌(Glom-erella cingulata Schr.et Spaulad)(由本所从苹果病果上分离得到)。 二、试验方法 1.药剂抑菌测定 采用菌落生长速率法进行室内药效测定。设50％多霉清试验浓度为800x、1000x、1200x、1500x,以70％甲基托布津800x药液为对照药剂,将药剂按一定倍数加入溶化的定量PDA培养基中,制作药剂培养基,
First, the test material 1 for the test agent: 50% mildew (Baoding Chemical Factory production), 70% thiophanate-methyl (Japan Soda Co., Ltd. production). 2. Species tested: Physalosproa piricola Nose, Glom-erella cingulata Schr.et Spaulad (isolated from apple fruits). Second, the test method 1 drug antibacterial assay using the colony growth rate method for the determination of indoor efficacy. Set 50% more mildew test concentration of 800x, 1000x, 1200x, 1500x, with 70% thiophanate-methyl 800x as the reference drug, the drug was added to the quantitative quantitative PDA medium dissolved in a certain amount of medium to produce pharmaceutical medium,