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目的了解2014年浙江省湖州市急性腹泻患者诺如病毒感染状况及基因特征,为加强感染性腹泻监测与综合防控工作提供依据。方法收集2014年湖州市综合性三级乙等和二级甲等各一家医院的粪便标本797份,采用实时荧光定量PCR方法进行诺如病毒核酸检测,并对诺如病毒核酸阳性标本进行衣壳蛋白区的序列测定和分析。结果 797份粪便标本中149份检出诺如病毒核酸阳性,阳性率18.70%(149/797),以GⅡ基因型为主,占89.26%(133/149)。衣壳蛋白区序列的系统进化分析显示检出的诺如病毒有5种GⅠ型别和7种GⅡ型别,包括GⅠ.1、GⅠ.2、GⅠ.5、GⅠ.6、GⅠ.7、GⅡ.2、GⅡ.3、GⅡ.4、GⅡ.6、GⅡ.8、GⅡ.17、GⅡ.21,以GⅡ.4 Sydney_2012(55.5%)、GⅡ.17(25.5%)型为主。结论诺如病毒在湖州市急性腹泻患者中存在较高感染,并且基因型别复杂,除了流行优势型别GⅡ.4外,2014年底还出现GⅡ.17型诺如病毒的流行,因此应加强诺如病毒腹泻监测工作,为今后疫情防控提供科学依据。
Objective To understand the status and gene characteristics of norovirus infection in patients with acute diarrhea in Huzhou City of Zhejiang Province in 2014 and provide basis for monitoring and comprehensive prevention and control of infectious diarrhea. Methods A total of 797 stool specimens collected from a total of Grade B and Grade A hospitals in Huzhou City in 2014 were collected. Real-time fluorescence quantitative PCR was used to detect Norovirus nucleic acid. Norovirus positive samples were encapsidated Sequence analysis and analysis of protein regions. Results 149 out of 797 stool specimens were positive for norovirus. The positive rate was 18.70% (149/797), accounting for 89.26% (133/149) of the G Ⅱ genotypes. Phylogenetic analysis of the capsid protein region sequence showed that there were 5 GⅠ and 7 GⅡ types detected in Norovirus, including GⅠ.1, GⅠ.2, GⅠ.5, GⅠ.6, GⅠ.7, GⅡ.2, GⅡ.3, GⅡ.4, GⅡ.6, GⅡ.8, GⅡ.17 and GⅡ.21 were mainly GⅡ.4 Sydney_2012 (55.5%) and GⅡ.17 (25.5%). Conclusions Norovirus is highly infectious in patients with acute diarrhea in Huzhou City, and the genotype is complex. In addition to the predominant genotype GⅡ.4, the genotype GⅡ.17 norovirus has also emerged at the end of 2014. Therefore, Such as virus diarrhea monitoring work, provide a scientific basis for the prevention and control of epidemic in the future.