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目的探讨同一居群滇龙胆根及根茎、茎、叶、花中裂环烯醚萜类主要活性成分的积累规律。方法采用高效液相色谱法(HPLC)测定同一居群滇龙胆根及根茎、茎、叶、花中龙胆苦苷、獐芽菜苦苷、獐芽菜苷等主要活性成分的含量,并建立其HPLC图谱,对滇龙胆不同部位中主要活性成分积累规律进行研究。结果同一居群滇龙胆根及根茎中龙胆苦苷含量极显著高于叶、花和茎(P<0.01),滇龙胆叶中獐芽菜苦苷含量极显著高于根及根茎、花、茎(P<0.01),滇龙胆花中獐芽菜苷含量显著高于根及根茎(P<0.05);同一居群滇龙胆根及根茎、茎、叶、花HPLC图谱相似度在0.986以上,确定HPLC图谱中26个共有峰。结论同一居群滇龙胆根及根茎、茎、叶、花中龙胆苦苷、獐芽菜苦苷、獐芽菜苷含量存在极显著(P<0.01)或显著(P<0.05)差异;滇龙胆根及根茎、叶、花HPLC图谱相似度较高,主要活性成分种类差异较小。
Objective To investigate the accumulation of the major active constituents of the secopenoid iridoid in the roots and roots, stems, leaves and flowers of Gentiana macrophylla from the same habitat. Methods The content of the major active components of gentian, rhubarb, glycoside and matrine in the same population of Radix Gentiana was measured by high performance liquid chromatography (HPLC) The HPLC profile was established to study the accumulation of active principle in different parts of Yunnan gentian. Results The content of gentiopicroside in the roots and rhizomes of Gentiana macrophylla was significantly higher than that in leaves, flowers and stems in the same population (P <0.01). The content of bitter glycosides in Gentiana macrophylla was significantly higher than that of roots and rhizomes, (P <0.01). The content of urotensin in Gentiana davurica was significantly higher than that in roots and rhizomes (P <0.05). The similarities were found between the same populations of Radix Gentianae and their roots, stems, leaves and flowers Above 0.986, 26 common peaks in the HPLC profile were identified. Conclusion There was a significant difference (P <0.01) or significant (P <0.05) differences in the contents of gentiopicroside, swertia glycosides and romaine in the roots, roots, stems, leaves and flowers of the same population. The HPLC fingerprints of Gentiana straminea, rhizome, leaf and flower of D. yunnanensis had higher similarity, but their main active components were less different.