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目的探讨重组质粒pZeoSV2(+)/CpG-HBcAg(ISS)对Balb/c小鼠免疫的作用。方法构建真核表达质粒pZeoSV2(+)/CpG-HBcAg(ISSb,ISSc),将其免疫Balb/c小鼠,ELISA法检测免疫后小鼠血清中HBcAb、IFN-γ、IL-2、IL-12、IL-4和IL-10的含量。结果 pZeoSV2(+)/CpG-HBcAg(ISSb,ISSc)重组质粒均能诱导Balb/c小鼠产生特异性抗体HBcAb,pZeoSV2(+)/CpG-HBcAg(ISSb)组较pZeoSV2(+)/CpG-HBcAg(ISSc)组产生的抗-HBc效价显著增高(P<0.01),且其免疫Balb/c小鼠后能使Th1型细胞因子IFN-γ、IL-2和IL-12的表达增强,抑制Th2型细胞因子IL-4和IL-10的产生。结论 pZeoSV2(+)/CpG-HBcAg(ISSb,ISSc)重组质粒对小鼠HBcAb的产生具有明显的促进作用,含CpG-HBcAg(ISSb)的重组质粒能够明显增强小鼠血清Th1型细胞因子的表达,抑制Th2型细胞因子的表达。
Objective To investigate the effect of recombinant plasmid pZeoSV2 (+) / CpG-HBcAg (ISS) on Balb / c mice. Methods The eukaryotic expression plasmid pZeoSV2 (+) / CpG-HBcAg (ISSb, ISSc) was constructed and immunized into Balb / c mice. The levels of HBcAb, IFN-γ, IL-2 and IL- 12, IL-4 and IL-10 content. Results The recombinant plasmid pZeoSV2 (+) / CpG-HBcAg (ISSb) could induce specific antibody HBcAb in Balb / c mice compared with pZeoSV2 (+) / CpG- The anti-HBc titer of HBcAg (ISSc) group was significantly increased (P <0.01), and the immunization of Balb / c mice enhanced the expression of Th1 cytokines IFN-γ, IL-2 and IL-12, Inhibit the production of Th2-type cytokines IL-4 and IL-10. Conclusion Recombinant plasmids pZeoSV2 (+) / CpG-HBcAg (ISSb, ISSc) can significantly promote the production of HBcAb in mice. Recombinant plasmids containing CpG-HBcAg (ISSb) can significantly enhance the expression of Th1 cytokines , Inhibit the expression of Th2 type cytokines.