Cloning and Expression of BTB/POZ-Domain from Zebrafish gcl （Germ Cell-Less）, and Appraisement of It

• 作 者: DENG Fengjiao CHENG Pengxiang
• 机 构: CollegeofLifeSciences,CenterforResearchonReproductionandWomen＇sHealth
• 出 处: 武汉大学学报：自然科学英文版
• 发表日期: 2008年1期
• 关键词: 发生遗传学 克隆技术 生物化学 多克隆技术 gel （germ cell-less） polycolonal-antibody BTB/ POZ domai
• 基金项目: Supported by the National Natural Science Foundation of China （30570968, 30370744）

In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl (germ cell-less) into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21(DE3) pLysS strain to express