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目的 :建立氯霉素氢化可的松滴耳液的含量测定方法。方法 :采用高效液相色谱法 ,SpherisorbC18(4.6mm× 2 5 0mm ,5 μm) ,流动相为甲醇 0 .0 2mol·L-1KH2 PO4(用磷酸调 pH至3.0 ) (6 0∶4 0 ) ,流速 0 .9ml·min-1,检测波长为 2 4 2nm。结果 :氯霉素和氢化可的松分别在 10 0 .2～ 4 0 0 .6 μg·ml-1和 2 0 .2～ 80 .6 μg·ml-1范围内线性关系良好 ,平均回收率分别为10 0 .2 % (RSD 0 .7% )、99.8% (RSD 0 .6 % )。结论 :本方法可同时测定两种成分含量 ,操作简便 ,结果准确。
Objective: To establish a method for determination of hydrocortisone ear drops. METHODS: Spherisorb C18 (4.6 mm × 250 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol 0. 2 mol·L -1 KH 2 PO 4 (adjusted to pH 3.0 with phosphoric acid) (60:40) , Flow rate 0. 9ml · min-1, detection wavelength of 224nm. Results: Chloramphenicol and hydrocortisone showed good linearity in the range of 10 0. 2 ~ 400 μg · ml -1 and 2 0. 2 ~ 80. 6 μg · ml -1, respectively. The average recovery They were 100.2% (RSD 0.7%) and 99.8% (RSD 0.6%), respectively. Conclusion: The method can simultaneously determine the content of two components, the operation is simple and the result is accurate.