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用抗原纯化和吸收剂处理患儿血清的方式，对已建立的间接酶联免疫吸附测定（ＥＬＩＳＡ）检测柯萨奇Ｂ组病毒（ＣＢＶ）－ＩｇＭ方法进行了改进，在确定其特异性、敏感性和可靠性的基础上，做了主要试剂的稳定性和抗破坏性试验证实符合商品试剂盒要求。２３７例病毒性心肌炎和４１例Ｉ型糖尿病患者血清经检测阳性率分别为３３．３％和５６．１％。本方法的阳性预测值为８０％，阴性预测值为１００％，变异系数小于１１．１％。主要试剂４℃保存半年或３７℃保存一周均对标本的检测结果无显著影响。
The method of detecting indirect coenzyme-linked immunosorbent assay (ELISA) for the detection of coxsackie virus B (CBV) -IgM was improved by means of antigen purification and absorbent treatment of pediatric serum. After determining its specificity and sensitivity On the basis of sex and reliability, the stability and destructiveness tests of the main reagents were done to confirm that they meet the requirements of the commercial kit. The positive rates of sera from 237 viral myocarditis patients and 41 patients with type 1 diabetes mellitus were 33.3% and 56.1%, respectively. The method has a positive predictive value of 80%, a negative predictive value of 100% and a coefficient of variation of less than 11.1%. The main reagents stored at 4 ℃ for half a year or 37 ℃ for one week have no significant effect on the test results.