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目的:探究新型表面活性剂分子Pa-Brij78在药物传递方面的应用。方法:利用Brij78合成Pa-Brij78,通过薄膜水化法得到3 m M包载姜黄素的Pa-Brij78胶束水溶液,并通过共沉淀法用磷酸钙将胶束矿化的流程建立磷酸钙胶束复合纳米颗粒药物传递系统;利用Pa-Brij78作为稳定剂,与紫杉醇按不同的紫杉醇/表面活性剂质量比例溶于氯仿后用氮气吹干,真空干燥2-4 h,薄膜物水化30 min后超声20 min的流程建立纳米晶体Pa-PNC。运用粒度仪和扫面电子显微镜对两种纳米系统进行表征。结果:磷酸钙胶束复合纳米颗粒粒径小于200 nm,纳米颗粒外貌都是球形且表面粗糙对姜黄素的载药率达17%,包封率大于90%,构建得到的不同比例的纳米晶体Pa-PNC,其粒径也都小于200 nm,Pa-PNC纳米晶体是棒状的,且Pa-PNC的载药率达50%。结论:本课题组合成的新型表面活性剂分子Pa-Brij78能够构建得到两种药物传递系统,在药物传递系统方面具有一定的应用潜力。
Objective: To explore the application of a novel surfactant molecule Pa-Brij78 in drug delivery. Methods: Pa-Brij78 was synthesized by Brij78, and 3 m M curcumin Pa-Brij78 micellar solution was obtained by membrane hydration method. The micellar micelles Composite nanoparticles drug delivery system; the use of Pa-Brij78 as a stabilizer, paclitaxel with different paclitaxel / surfactant mass ratio dissolved in chloroform after blowing dry with nitrogen, vacuum drying 2-4 h, the film hydration 30 min Ultrasound 20 min process to establish nanocrystalline Pa-PNC. Both nanosystems were characterized using a particle sizer and a scanning electron microscope. Results: The particle size of calcium phosphate micellar composite nanoparticles was less than 200 nm. The appearance of the nanoparticles was spherical and the surface roughening rate was 17% for curcumin. The entrapment efficiency was more than 90%. Different proportions of nanocrystals Pa-PNC, the particle size is also less than 200 nm, Pa-PNC nanocrystals are rod-shaped, and Pa-PNC drug loading rate of 50%. Conclusion: The novel surfactant molecule Pa-Brij78 synthesized by this research group can be used to construct two drug delivery systems and has potential application in drug delivery system.