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目的研究成年小鼠自反应Ｔ细胞的清除机制，阐明成年小鼠自身耐受的发生方法胸腺摘除成年小鼠静脉注射ＭＬｓ＋的活化Ｂ细胞．在注射后不同时间点，利用流式细胞技术观察淋巴结中ＣＤＶβ６＋ＣＤＶβ８＋、ＣＤＶβ６＋、和ＣＤＶβ８＋细胞百分比的变化，局部ＧＶＨＲ和抗活化Ｂ细胞混合淋巴细胞反应．结果在胸腺摘除的成年小鼠中静脉注射ＭＬｓ＋的活化Ｂ细胞可诱导ＭＬｓ特异性免疫耐受．来源于ＣＤＦ１小鼠的脾脏Ｂ淋巴细胞经抗ＩｇＭ抗体加ＩＬ—４刺激，静脉注射给摘除胸腺的成年ＢＡＬＢ／Ｃ小鼠（ＭＬ－Ｉｂ）后第７、１１、１５天，淋巴结中ＣＤＶβ６＋细胞显著减少，而ＣＤＶβ８＋细胞数无明显改变；在注射后第３无ＣＤＶβ６＋细胞增加，但对ＭＬＳ抗原的反应性明显低于对照组．结论自反应Ｔ细胞不但在胸腺被排除或功能性“排除”，而且在外周通过同样的机制维持自身耐受．
OBJECTIVE: To study the mechanism of self-reactive T cell depletion in adult mice and to elucidate the mechanism of self-tolerance in adult mice. Thymus-excised adult mice were injected with MLs + activated B cells intravenously. At different time points after injection, the changes of percentage of CDVβ6 + CDVβ8 +, CDVβ6 +, and CDVβ8 + cells in lymph nodes were observed by flow cytometry. Local GVHR and anti-activated B cells mixed lymphocyte reaction. Results Intravenous injection of MLs + activated B cells in thymus-evoked adult mice induces MLs-specific immune tolerance. Splenic B lymphocytes derived from CDF1 mice were stimulated with anti-IgM antibody plus IL-4 and injected intravenously to the thymus-depleted adult BALB / C mice (ML-Ib) on days 7, 11 and 15, and lymph node CDVβ6 + cells While the number of CDVβ8 + cells did not change significantly. No CDVβ6 + cells increased after injection, but the response to MLS antigen was significantly lower than that of the control group. CONCLUSIONS: Self-reactive T cells are not only excluded or functionally excluded in the thymus, but also maintained self-tolerance in the periphery by the same mechanism.