论文部分内容阅读
目的探讨乙肝产妇血清中HBV-DNA含量与母乳HBV-DNA含量的关系,以便更好的指导母乳喂养。方法采用实时定量PCR(Real-time quantitative PCR,RQ-PCR)方法,对80例乙肝血清学阳性的产妇血清及乳汁中HBV-DNA定量检测,分析血清HBV-DNA含量和乳汁HBV-DNA含量之间的相关关系。结果80例患者中,60例血清HBV-DNA检测阳性,其中HBV-DNA含量>1×105IU/ml为39例,血清HBV-DNA>1×105IU/ml的39例患者,有30人乳汁中检测到HBV-DNA,阳性率为76.92%,而且乳汁中HBV-DNA含量对数值与血清HBV-DNA含量对数值有正相关关系,相关系数r=0.924(r=0.924);≤1×105IU/ml为21例,血清HBV-DNA≤1×105IU/ml的21例患者,乳汁中均未检测到HBV-DNA,乳汁HBV-DNA阳性患者,血清HBeAg(+)占80%,HBeAg(-)占20%,两组比较,差异有统计学意义(P<0.01);两组患者乳汁HBV-DNA含量无显著性差异(P>0.05)。结论产妇血清与乳汁中HBV-DNA含量具有密切的相关性,而血清中的HBeAg与乳汁中HBV-DNA含量无明显关系,血清中的HBeAg作为产妇哺乳传染性指标,尚有待进一步研究。
Objective To investigate the relationship between HBV-DNA in serum of hepatitis B patients and the content of HBV-DNA in breast milk in order to better guide the breast-feeding. Methods Real-time quantitative PCR (RQ-PCR) was used to detect HBV-DNA in serum and milk of 80 cases of seropositive hepatitis B patients. Serum HBV-DNA content and serum HBV-DNA content The correlation between. Results Among the 80 patients, the serum HBV-DNA was positive in 60 cases, of which 39 cases were HBV-DNA> 1 × 105 IU / ml and 39 cases were serum HBV-DNA> 1 × 105 IU / ml. The positive rate of HBV-DNA was 76.92%, and the positive correlation between the value of HBV-DNA in breast milk and serum HBV-DNA had a positive correlation (r = 0.924, r = 0.924) 21 cases with serum HBV-DNA≤1 × 105IU / ml, no HBV-DNA and breast milk HBV-DNA positive were detected in serum. Serum HBeAg (+) accounted for 80%, HBeAg (-) Accounting for 20%. There was significant difference between the two groups (P <0.01). There was no significant difference in serum HBV-DNA between the two groups (P> 0.05). Conclusions There is a close correlation between serum HBV-DNA in breast milk and maternal serum. There is no significant relationship between serum HBeAg and HBV-DNA in breast milk. HBeAg in serum is an infectious indicator of maternal lactation, which remains to be further studied.