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目的 :探索釉基质蛋白 (EMPs)促进成骨细胞增殖分化的作用 ,为进一步研究提供基本数据。方法 :选择MC3T3 E1成骨细胞株 ,利用体外细胞培养方法 ,分别加入不同浓度的EMPsα MEM培养液 ,于培养 0、1、2、3、4,5、6d进行细胞计数 ,采用SAS软件对细胞计数数据作单因素方差分析。结果 :EMPs各组细胞计数均高于阴性对照 (C)组 (P <0 0 1)。第 2天 ,10 0 μg/ml和 15 0 μg/mlEMPs组的细胞计数高于阳性对照 (T)组 (P <0 0 1) ;第 5天 ,10 0μg/mlEMPs组的细胞计数不但高于C、T组 ,还明显高于其它EMPs浓度组。结论 :釉基质蛋白可明显促进成骨细胞的增殖分化。
Objective: To explore the effect of enamel matrix proteins (EMPs) on the proliferation and differentiation of osteoblasts and provide basic data for further study. Methods: MC3T3 E1 osteoblasts were selected and cultured in vitro with different concentrations of EMPsα MEM. The cells were counted on 0, 1, 2, 3, 4, 5 and 6 days after culture. SAS software Count data for one-way analysis of variance. Results: The cell counts of EMPs in each group were higher than those in negative control group (P <0.01). On the second day, the cell counts of 10 0 μg / ml and 150 μg / mlEMPs group were higher than those of the positive control (T) group (P <0.01). On the 5th day, the cell counts of 10 μg / mlEMPs group were not only higher than C, T group, but also significantly higher than other EMPs concentration group. Conclusion: Enamel matrix protein can significantly promote the proliferation and differentiation of osteoblasts.