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目的：探讨ＡＦＰｍＲＮＡ的表达与ＨＢＶ感染的关系。方法：采用逆转录ＰＣＲ技术对３１例原发肝癌组织中ＡＦＰｍＲＮＡ的表达进行了观察。结果：３１例肝癌组织有２６例（８３．９％）检出ＡＦＰｍＲＮＡ，阳性者中有７例同时伴有癌旁肝组织ＡＦＰｍＲＮＡ的表达，此７例患者均伴有明显的肝硬化。肝癌组织ＡＦＰｍＲＮＡ检出率在血清ＨＢｓＡｇ阳性组（８６．９％），伴有肝硬化组（８７．０％）和血清ＡＦＰ＞３０ｎｇ／ｍｌ组（１００％）分别显著高于血清ＨＢｓＡｇ阴性组（５０％）、无伴有肝硬化组（５０．０％）和血清ＡＦＰ≤３０ｎｇ／ｍｌ组（４６．０２％）（Ｐ＜０．０５～０．０１）。结论：肝癌组织ＡＦＰｍＲＮＡ的表达可能与ＨＢＶ感染，肝细胞再生修复有关。
Objective: To investigate the relationship between AFP mRNA expression and HBV infection. Methods: The expression of AFP mRNA in 31 cases of primary hepatocellular carcinoma was observed by reverse transcription PCR. Results: AFP mRNA was detected in 26 cases (83.9%) of 31 cases of hepatocellular carcinoma, and 7 cases of positive tumors were also associated with the expression of AFPmRNA in the adjacent liver tissues. All 7 cases were accompanied with obvious liver cirrhosis. The detection rate of AFP mRNA in HCC tissues was significantly higher in serum HBsAg positive group (86.9%), cirrhosis group (87.0%) and serum AFP>30 ng/ml group (100%) than serum HBsAg negative group ( 50%), no cirrhosis group (50.0%) and serum AFP ≤ 30 ng/ml group (46.02%) (P<0.05-0.01). Conclusion: The expression of AFP mRNA in hepatocellular carcinoma may be related to HBV infection and regeneration of hepatocytes.