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由于新鲜软骨来源有限,有必要建立异体移植软骨库。冷冻保存法能使软骨细胞长期存活并维持其正常的代谢和生物学特性。鼻中隔软骨取自重建手术中或尸体材料,共15例,年龄5～80岁。经消化酶消化、孵育、过滤。从标本中的软骨中分离软骨细胞,进行细胞的计数,用台盼蓝染料测定细胞活性。以不同培养基孵育分离出的软骨细胞,倒置显微镜观察不同孵育期软骨细胞增殖情况。上述细胞经冷冻
Due to the limited availability of fresh cartilage, it is necessary to establish a xenograft cartilage reservoir. Cryopreservation allows chondrocytes to survive long-term and maintain their normal metabolic and biological properties. Nasal septal cartilage was taken from reconstructive surgery or cadaver material, a total of 15 cases, aged 5 to 80 years. Digested by digestive enzymes, incubated and filtered. Chondrocytes were isolated from the cartilage in the specimen, and the cells were counted, and the cell viability was measured by trypan blue dye. Chondrocytes isolated from different culture media were incubated, and the proliferation of chondrocytes at different incubation periods was observed under inverted microscope. The above cells are frozen