论文部分内容阅读
以氯化镉、碲氢化钾为原料,巯基乙酸钠为包裹剂水相合成了碲化镉纳米晶,通过透射电镜、高分辨透射电镜、能量色散谱、荧光光谱、紫外可见光谱、X射线衍射对碲化镉纳米晶进行了表征,所合成的纳米晶直径为5nm左右,属立方结构.碲化镉纳米晶溶液在制备后放置19d其荧光量子产率从初始的37%达到97%,而最大荧光发射波长(λem)从543nm移至510nm,蓝移33nm,而且在冰箱4℃可稳定至少10个月.同时碲化镉纳米晶溶液也具有一定的共振瑞利散射(RRS),其最大散射峰位于554nm附近.研究了碲化镉纳米晶与硫酸阿米卡星和硫酸小诺霉素的相互作用对荧光和RRS的影响.结果表明:当两者反应形成结合产物时,将使纳米晶溶液荧光显著猝灭并使RRS明显增强,并且荧光猝灭作用和RRS增强在一定的范围内与药物的浓度成正比,对于硫酸阿米卡星和硫酸小诺霉素的检出限分别为3.4和2.6ng/mL(荧光猝灭法)及15.2和14.0ng/mL(RRS法),方法具有高灵敏度.因此为用碲化镉纳米晶作荧光探针荧光猝灭法和RRS法测定氨基糖苷类药物创造了条件.
The CdTe nanocrystals were synthesized by using cadmium chloride and potassium telluride as raw materials and sodium thioglycolate as the encapsulating agent in water. The nanocrystals were characterized by transmission electron microscopy, high resolution transmission electron microscopy, energy dispersive spectroscopy, fluorescence spectroscopy, UV-visible spectroscopy, X-ray diffraction The CdTe nanocrystals were characterized by a cubic structure with a diameter of about 5 nm.The fluorescence quantum yield of cadmium telluride nanocrystals reached 97% from the initial 37% after being prepared for 19 d The maximum fluorescence emission wavelength (λem) was shifted from 543nm to 510nm, the blue shift was 33nm, and stable in the refrigerator at 4 ℃ for at least 10 months.At the same time CdTe nanocrystal solution also has some Resonance Rayleigh scattering (RRS) The scattering peak is near 554nm.The influence of the interaction between cadmium telluride nanocrystal and amikacin sulfate and microtomycin sulfate on the fluorescence and RRS is studied.The results show that when the two react to form a binding product, Crystal fluorescence significantly quenched and RRS significantly enhanced, and fluorescence quenching and RRS enhancement in a certain range with the concentration of the drug is proportional to the detection limits of amikacin sulfate and nitric acid sulfate were 3.4 and 2.6 ng / mL (Fluorescence Quenching method) and 15.2 and 14.0ng / mL (RRS method), a method having high sensitivity. Thereby creating the conditions for measurement using aminoglycosides CdTe nanocrystals as fluorescent probe and fluorescence quenching method RRS method.