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目的 探讨抗中性粒细胞浆抗体 (ANCA)在妊娠高血压综合征 (妊高征 )患者内皮细胞损伤中的作用及可能机制。方法 采用间接免疫荧光法 (IIF)和酶联免疫吸附试验 (EL ISA)对 32例中重度妊高征患者作血中 ANCA的定性和定量检测 ,并以 44例正常孕妇作对照。结果 32例妊高征患者 ANCA阳性者 13例 (胞浆型 ANCA阳性 9例 ,核周型阳性 4例 ) ,正常孕妇无 1例 ANCA阳性。妊高征患者 ANCA含量 0 .45± 0 .18g/ L,正常孕妇为 0 .2 3± 0 .0 9g/ L,两组间定性及定量差异均有显著性 (P<0 .0 5 )。结论 ANCA可能参与妊高征患者血管内皮细胞损伤机制。
Objective To investigate the role and possible mechanism of anti-neutrophil plasma antibody (ANCA) in endothelial cell injury in patients with pregnancy induced hypertension (PIH). Methods The qualitative and quantitative detection of ANCA in blood of 32 patients with moderate and severe pregnancy induced hypertension were detected by indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA), and 44 normal pregnant women were used as controls. Results Thirty-two ANCA positive cases were found in 32 cases of PIH (cytoplasmic ANCA positive in 9 cases and perinuclear positive in 4 cases). None of the normal pregnant women had positive ANCA. Patients with PIH had an ANCA level of 0.45 ± 0.18g / L and a normal pregnant women of 0.23 ± 0.99g / L, with significant differences between the two groups in qualitative and quantitative (P <0.05) . Conclusion ANCA may be involved in vascular endothelial cell injury in patients with PIH.