论文部分内容阅读
1 材料与方法 1.1检测对象:本院医疗区41部电话送话器。 1.2取样:将无菌棉拭子浸入盛有0.6ml无菌生理盐水的Eppendorf管中,湿润后取出,擦拭送话器表面,然后再将棉拭子浸入管中搅动片刻,弃去拭子,把样品保存于-20℃待检。 1.3仪器:美国PERKIN ELMER公司生产的GeneAmp PCR System 9600。 1.4试剂;使用军事医学科学院放射医学研究所生产的乙型肝炎病毒脱氧核糖核酸聚合酶链反应(英文缩写为HBV-DNA PCR)试剂盒。 1.5实验方法:按仪器及试剂盒说明书进行操
1 Materials and Methods 1.1 test object: Hospital medical area 41 telephone microphones. 1.2 Sampling: The sterile cotton swab immersed in 0.6ml sterile saline Eppendorf tube, wet out, wipe the microphone surface, and then the cotton swab immersed in the tube and stirred for a moment, discard the swab, Store the samples at -20 ° C for inspection. 1.3 Instrument: the United States PERKIN ELMER GeneAmp PCR System 9600. 1.4 reagents; use of the Institute of Radiation Medicine Military Academy of Medical Sciences produced by Hepatitis B virus DNA polymerase chain reaction (abbreviated as HBV-DNA PCR) kit. 1.5 Experimental method: according to the instructions of the instrument and kit operation