论文部分内容阅读
目的比较以CD105和CD34为血管内皮细胞标记物在早期直肠癌新生血管中表达的差异,探讨CD105-微血管密度(MVD)作为Ⅰ-Ⅱ期直肠癌预后预测指标的可能性。方法分别应用抗CD105、CD34抗体对380例Ⅰ-Ⅱ期直肠癌组织微血管进行免疫组织化学标记,多因素回归分析CD105-MVD、CD34-MVD与早期直肠癌肝转移及预后的相关性。结果CD105在直肠癌新生微血管中特异性表达,在正常组织的血管无表达,而CD34在肿瘤及正常组织的大小血管中均广泛表达,两者表达率有显著性差异(P<0.001);CD105-MVD与肿瘤浸润深度、病理组织类型、肝转移率呈正相关。多因素分析表明CD105-MVD是影响全组直肠癌预后的独立因素,CD105-MVD高表达时患者死亡的危险度是低表达患者的3.032倍(95%CI=1.481-6.804)。而CD34-MVD与直肠癌的预后无明显相关性。结论CD105是一种较CD34更有特异性的肿瘤性血管内皮细胞标记物,CD105抗体标记的肿瘤微血管密度可作为预测早期直肠癌预后的重要分子生物学指标。
Objective To compare the expression of CD105 and CD34 as markers of vascular endothelial cell in early stage of rectal neoplasms and explore the possibility of CD105-MVD as a predictor of prognosis of stage Ⅰ-Ⅱ rectal cancer. Methods The anti-CD105 and CD34 antibodies were respectively used to detect the microvessel density in 380 cases of stage Ⅰ-Ⅱ colorectal cancer. The correlation between CD105-MVD, CD34-MVD and liver metastasis and prognosis of early rectal cancer was analyzed by multivariate regression analysis. Results CD105 was specifically expressed in neovascularization of rectal cancer, but no expression was found in normal blood vessels. CD34 was widely expressed in the size and size of tumor and normal tissues, with a significant difference (P <0.001). CD105 -MVD and tumor invasion depth, pathological type, liver metastasis rate was positively correlated. Multivariate analysis showed that CD105-MVD was an independent factor affecting the prognosis of rectal cancer in all groups. The high risk of death from CD105-MVD was 3.032 times (95% CI = 1.481-6.804) in patients with low expression of CD105-MVD. However, there was no significant correlation between CD34-MVD and prognosis of rectal cancer. Conclusion CD105 is a more specific tumor vascular endothelial cell marker than CD34. The density of CD105 labeled tumor microvessels can be used as an important molecular biological index to predict the prognosis of early rectal cancer.