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目的 探讨恶性肿瘤患者肿瘤组织细胞DNA倍体类型与细胞动力学参数之间的关系。方法 采用流式细胞术对2 5 0 4例恶性肿瘤组织DNA含量进行了检测 ,对DNA倍体进行了分类 ,并分析了DNA倍体类型与细胞凋亡 (Apo)检出率及S期细胞比率 (SPF)的关系。结果 恶性肿瘤组织DNA倍体分型 :二倍体 (D ,473例 )、近二倍体 (ND ,49例 )、四倍体 (T ,3 0 7例 )、非整倍体 (AN ,10 40例 )和多异倍体 (M ,63 5例 )。后 4种统称为DNA异倍体 (H )共 2 0 3 1例。恶性肿瘤组织Apo检出率显著低于正常对照组 (P <0 .0 1) ,而SPF比率却显著高于正常对照组 (P <0 .0 1)。二倍体肿瘤组织Apo检出率显著高于异倍体肿瘤组织 (P <0 .0 1) ,SPF比率显著低于异倍体组织 (P <0 .0 1)。在恶性肿瘤组织中 ,含有不同数量异倍体克隆者、不同DNA倍体异质性者的Apo检出率和SPF比率均有显著性差异 (P <0 .0 1)。不同DI值的肿瘤组织其Apo检出率和SPF比率有明显不同。 结论 恶性肿瘤能导致肿瘤组织Apo水平显著降低 ,细胞增殖活性显著增加。而且这些变化与肿瘤组织倍体类型、DI值、DNA异倍体克隆数以及DNA倍体异质性等均有十分密切的关系。
Objective To investigate the relationship between DNA ploidy types and cell kinetic parameters of tumor cells in patients with malignant tumors. Methods The DNA content of 2504 malignant tumor tissues was detected by flow cytometry. DNA ploidy was classified and the DNA ploidy type and the detection rate of apoptosis (Apo) and S phase cells Ratio (SPF) relationship. Results DNA ploidy types of malignant tumors were diploid (D, 473), nearly diploid (ND, 49), tetraploid (T, 307), aneuploid (AN, 10 40 cases) and polyploidy (M, 63 5 cases). The latter 4 collectively referred to as DNA aneuploidy (H) a total of 203 cases. The detection rate of Apo in malignant tumor tissue was significantly lower than that in normal control group (P <0.01), while the SPF rate was significantly higher than that in normal control group (P <0.01). The detection rate of Apo in diploid tumor tissues was significantly higher than aneuploid tumor tissues (P <0.01), and the SPF ratio was significantly lower than aneuploid tissues (P <0.01). In malignant tumors, the detection rates of Apo and SPF were significantly different among those with different numbers of aneuploid clones and different DNA ploidy heterogeneity (P <0.01). Different DI value of the tumor tissue Apo detection rate and SPF ratio were significantly different. Conclusion Malignant tumors can cause Apo levels in tumor tissue to decrease significantly and cell proliferation activity to increase remarkably. And these changes are closely related to tumor tissue type, DI value, DNA aneuploidy and DNA ploidy heterogeneity.