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目的:通过对蒙古黄芪植株各部位提取液的生物学试验,探讨蒙古黄芪是否存在自毒作用,以及自毒作用与连作障碍的关系。方法:设置蒸馏水(CK)、蒙古黄芪根、茎和叶水浸提液各12.5、25、50和100 mg/mL 4个浓度,共计13个处理,用于黄芪自毒作用的生物学实验。结果:当蒙古黄芪根、茎和叶水浸提液的浓度达到25 mg/mL时,对自身种子萌发和幼苗生长有抑制作用,且随水浸提液浓度的升高,这种抑制作用增强;就综合化感效应而言,茎水浸提液的抑制作用>叶水浸提液>根水浸提液。发芽指数和幼苗活力指数对水浸提液比其他指标更为敏感。结论:蒙古黄芪植株水浸提液对自身种子萌发和幼苗生长具有明显的抑制作用,且抑制作用具有一定的浓度依赖性,蒙古黄芪自毒作用是造成连作障碍的原因之一。
OBJECTIVE: To investigate the existence of autotoxicity of Astragalus mongholicus and the relationship between autotoxicity and continuous cropping obstacle by biological experiments on the extracts of Radix Astragali from different parts of Mongolia. Methods: Four concentrations of distilled water (CK), Astragalus mongolica root, stem and leaf water extracts at concentrations of 12.5, 25, 50 and 100 mg / mL were prepared for biological experiments. Results: When the concentration of the extract of Astragalus root, stem and leaf in Mongolia reached 25 mg / mL, the seed germination and seedling growth were inhibited. With the increase of water extract concentration, the inhibitory effect was enhanced ; For the combined effect of the allelopathic effect, the inhibitory effect of stem water extract> leaf water extract> root water extract. Germination index and seedling vigor index were more sensitive to aqueous extract than other indexes. CONCLUSION: The aqueous extract of Astragalus membranaceus from Mongolia had a significant inhibitory effect on its own seed germination and seedling growth, and the inhibitory effect was in a concentration-dependent manner. Mongolian astragalus self-toxicity was one of the causes of continuous cropping obstacle.