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目的 :观察参芪扶正注射液对实验性糖尿病大鼠肾脏的保护作用。方法 :雄性SD大鼠 30只 ,用 0 1mol/L枸橼酸缓冲液溶解链脲佐菌素 (streptozotocin ,STZ) ,以 6 0mg/kg给予大鼠腹腔注射制造糖尿病大鼠模型。 2 4只成功的模型分为糖尿病组 (n =12 ) ;糖尿病治疗组 (n =12 ) ,给予参芪扶正注射液 2 5 0ml/(kg·d)口服。另选 8只为正常组 ,腹腔注射等量枸橼酸缓冲液。各组不应用降糖药。观察第 4周 ,8周 2 4h尿蛋白定量、平均动脉收缩压、第 8周血肌酐 (Scr)、尿素氮 (BUN)、肾重 /体重、血管紧张素Ⅱ (AngⅡ )含量及肾组织转化生长因子 β(TGF β1)、原癌基因蛋白c Fos的表达 ,并应用计算机图象分析系统测定其表达的灰度值。结果 :治疗组 2 4h尿蛋白含量 4周 ,8周 (2 4 37± 7 83)mg ,(2 7 76± 8 6 2 )mg均明显低于糖尿病组 (31 0 2± 9 32 )mg ,(36 10± 12 2 6 )mg (P <0 0 5 ) ;平均动脉收缩压治疗组 (114 33± 7 6 3)mmHg(1mmHg =0 .133kPa) ,(12 0 0 0± 7 46 )mmHg显著低于糖尿病组 (132 33±8 5 2 )mmHg ,(139 6± 9 6 7)mmHg (P <0 0 1) ;AngⅡ含量治疗组 (184 82± 6 1)ng/L明显低于糖尿病组 (2 83 6 1±78 0 6 )ng/L(P <0 0 1) ;Scr、BUN含量治疗组 (75 4± 2 7 1) μmol/L ,(9 87± 2
Objective: To observe the protective effect of Shenqi Fuzheng injection on the kidney in experimental diabetic rats. Methods: Thirty male Sprague-Dawley rats were randomly divided into three groups: control group, STZ (0 1 mol / L citrate buffer) and intraperitoneal injection of 60 mg / kg. Twenty-four successful models were divided into diabetic group (n = 12) and diabetes mellitus group (n = 12), and received Shenqi Fuzheng Injection 250ml / (kg · d) orally. Another eight for the normal group, intraperitoneal injection of citric acid buffer. Each group does not use hypoglycemic agents. Urinary protein, average arterial pressure, Scr, BUN, renal weight / body weight and angiotensin Ⅱ content in 24 hours and 8 weeks were observed, The expression of TGFβ1 and proto-oncogene c Fos were detected by computer image analysis system. Results: The proteinuria of urine in 24 hours, 24 weeks, 8 weeks (24 7 ± 7 83) mg and (27 76 ± 8 6 2) mg in the treatment group were significantly lower than those in the diabetic group (31 0 2 ± 9 32) mg, (36 10 ± 12 2 6) mg (P 0 05). Mean systolic blood pressure (114 33 ± 7 6 3) mmHg (1 mmHg = 0.133 kPa) and (12 0 0 ± 7 46) mmHg (132 33 ± 8 5 2) mmHg, (139 6 ± 9 6 7) mmHg (P 0 01) in diabetic group and 184 82 ± 6 1 ng / L in diabetic group were significantly lower than those in diabetic group (2 83 6 1 ± 78 0 6) ng / L (P 0 01). The levels of Scr and BUN in the treatment group were (75 4 ± 2 7 1) μmol / L, (9 87 ± 2)