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目的阐明人呼吸道合胞病毒(Human Respiratory Syncytial Virus,HRSV)A、B亚型病毒分离株的基质蛋白(MatrixProtein,M)编码基因特征。方法采用逆转录-聚合酶链反应(Reverse Transciption-Polymerase Chain Reaction,RT-PCR)对北京市2004年分离的HRSV代表株(A、B亚型各2株)进行M基因全长序列扩增、测序,并和基因库下载的所有HRSV的M基因全长序列进行对比和分析。结果2株HRSVA亚型分离株与A亚型Long株(标准株)M基因之间的核苷酸和氨基酸差异,分别为24、26个(3.1%～3.4%)和1个(0.39%)。2株HRSVB亚型分离株与B亚型参考株9320的M基因之间的核苷酸和氨基酸差异,分别为16、19个(2.1%、2.46%)和2、1个(0.78%、0.39%)。所测定的北京市4株HRSV分离株和从基因库下载的M基因全序列之间的核苷酸和氨基酸差异,分别为24～119个(3.1%～15.4%)和1～21个(0.39%～8.2%)。结论M基因在HRSV基因组中是较为保守的基因之一,A或B亚型的型内差异相对较小;但A、B亚型之间有较大差异,变异平均分配于整个M基因中。M基因的这些基因特征使其可以作为A、B亚型基因检测分型诊断的候选靶基因。该研究为HRSV基因快速诊断试剂的研制提供了基因信息学数据。
Objective To elucidate the gene encoding Matrix Proteins (M) gene of human respiratory syncytial virus (HRSV) subtype A and B isolates. Methods The full-length M gene was amplified from the representative strain of HRSV isolated from Beijing in 2004 (2 strains of A and B subtypes) by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Sequenced, and compared to the full length M sequences of all HRSVs downloaded from the GenBank. Results The nucleotide and amino acid differences between the two HRSVA isolates and the M subtype of the A subtype A strain (standard strain) were 24, 26 (3.1% -3.4%) and 1 (0.39% . The nucleotide and amino acid differences between the two strains of HRSVB isolates and the M gene of subtype B reference strain 9320 were 16,19 (2.1%, 2.46%) and 2,1 (0.78%, 0.39 %). The nucleotide and amino acid differences between the sequences of the four HRSV isolates in Beijing and the M sequences downloaded from the gene bank were 24 to 119 (3.1% to 15.4%) and 1 to 21 (0.39 % ~ 8.2%). Conclusion The M gene is one of the more conserved genes in the HRSV genome. The intraspecific difference of A or B subtypes is relatively small. However, the subtypes A and B are greatly different, and the variation is evenly distributed in the entire M gene. These gene characteristics of M gene make it possible to be used as a candidate target gene for typing diagnosis of subtype A and subtype B genes. This study provides genetic informatics data for the development of HRSV rapid diagnostic reagents.