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黄连为常用中药之一,收载于《中华人民共和国药典》1977年版一部中。规定本品含小檗碱按干燥品计算不得少于4.0%。近年来的研究表明:黄连中除小檗碱外,巴马亭和药根碱也有明显的抗菌作用。今对国内各种商品黄连中三种生物碱进行含测比较,以提供质量鉴别的依据。含量测定精密称取过60目筛的样品粉末0.1克,甲醇回流提取。用改良碘化铋钾试剂检查至无生物碱反应时停止回流。过滤液浓缩。浓缩液定量转移至1毫升容量瓶中,甲醇稀释至刻度,备用。取一定量上述溶液点样于硅
Coptis is one of the commonly used traditional Chinese medicines and is contained in the 1977 edition of the “Pharmacopoeia of the People’s Republic of China”. It is stipulated that the product containing berberine should not be less than 4.0% on a dry product basis. In recent years, studies have shown that in addition to berberine in Rhizoma Coptidis, bamatin and jatrorrhizine also have significant antibacterial effects. This paper compares the three alkaloids in various domestic products of Coptis chinensis in order to provide the basis for quality identification. Determination of Content 0.1 g of a sample powder finely weighed in a 60-mesh sieve was weighed and extracted with methanol under reflux. The reflux was stopped when using a modified potassium iodide reagent until no alkaloid reaction occurred. The filtrate is concentrated. The concentrate was quantitatively transferred to a 1 ml volumetric flask and diluted with methanol to the mark until ready. Take a certain amount of the solution spotted on silicon