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目的探讨以树突状细胞(DC)为介导的原发性肝癌免疫治疗新方法。方法采用肝癌细胞制备肿瘤细胞性抗原冲击致敏体外培养的DC,体外混合淋巴细胞反应检测抗原致敏DC刺激同基因T淋巴细胞增殖的能力,观察负载肿瘤抗原的DC体内外诱导产生肿瘤特异性细胞毒T淋巴细胞(CTL)及其抗肿瘤能力。结果经肝癌细胞抗原致敏的DC能诱导较强的自体T细胞增殖,且能诱导特异性CTL,该CTL对自体肝癌细胞具有很强的杀伤活性,杀伤率明显高于DC和未经肝癌细胞抗原致敏的DC激活的CTL及T淋巴细胞的杀伤率,而对非同种肿瘤细胞无明显的杀伤作用,经BEL7402肿瘤细胞抗原致敏的DC经皮下免疫小鼠可诱导有效的免疫保护作用,可抵抗野生型BEL7402细胞的再攻击,肿瘤生长受到明显抑制,瘤体出现延迟,生长减慢。结论DC具有强大的刺激T淋巴细胞增殖和诱导产生特异性CTL的能力,在体内外均能激发特异性抗肿瘤免疫应答。
Objective To explore a new method of immunotherapy for primary hepatocarcinoma mediated by dendritic cells (DCs). Methods Tumor cell antigen-sensitized DCs were cultured in vitro. The mixed lymphocyte reaction in vitro was used to detect the ability of antigen-sensitized DCs to stimulate the proliferation of syngeneic T lymphocytes. The tumor-specific expression of tumor-specific DCs was observed in vitro and in vivo Cytotoxic T lymphocytes (CTLs) and their anti-tumor ability. Results DCs sensitized by hepatoma cells could induce stronger proliferation of autologous T cells and induce specific CTLs. The CTLs had strong cytotoxic activity on autologous hepatocarcinoma cells, and the cytotoxicity was significantly higher than that of DCs and without hepatoma cells Antigen-induced DC-activated CTL and T lymphocyte killing rate, but non-allogeneic tumor cells had no significant killing effect, BEL7402 tumor antigen-primed DC subcutaneously immunized mice induced an effective protective effect , Can resist the re-attack of wild-type BEL7402 cells, tumor growth was significantly inhibited, the tumor appeared delayed, slowed down. Conclusion DC possesses strong ability of stimulating T lymphocyte proliferation and inducing specific CTL, and can stimulate specific antitumor immune response both in vitro and in vivo.