论文部分内容阅读
在甘氨酸-乙酸缓冲系统(pH 3.10)下采用9％聚丙烯酰胺凝胶电泳,比较研究了小麦(Triticumaestivum L.)“烟农15”、“济南13”、“莱州95”和“Marquis”品种的醇溶蛋白图谱误差变化及其有关控制方法。在每个凝胶板上误差呈规律性变化,迁移距离越大,谱带误差越大。同2.05cm谱带相比,9.25cm谱带标准误为0.0332,增大2倍;极差由1.5mm增大到4.5mm。国内3个小麦品种“莱州953”、“济南13”和“烟农15”群体内不存在电泳生物型。“莱州953”由于深色谱带多、分布合理,可作为基本电泳参照品种;“济南13”则由于总带数和特有谱带多,可作辅助参照品种。根据筛选的2.05、3.15、6.05和9.25cm标志带及其实际迁移大小,计算各电泳道谱带的矫正系数和矫正迁移距离。这种移动矫正法能使图谱误差降低约2/3。
9% polyacrylamide gel electrophoresis was carried out under the glycine-acetic acid buffer system (pH 3.10). The effects of different concentrations of Triticum aestivum L. on “Yannong 15”, “Jinan 13”, “Laizhou 95” and “Marquis” Changes of gliadin profile error and related control methods. The error in each gel plate changes regularly, the greater the migration distance, the greater the spectral error. Compared with 2.05cm band, 9.25cm band standard error of 0.0332, an increase of 2 times; range from 1.5mm to 4.5mm. There are no electrophoretic biotypes in the domestic three wheat cultivars “Laizhou 953”, “Jinan 13” and “Yannong 15”. “Laizhou 953” can be used as basic electrophoresis reference species due to its large number of dark bands and reasonable distribution. “Jinan 13” can be used as an auxiliary reference species due to its large total number of bands and unique bands. According to the selected 2.05, 3.15, 6.05 and 9.25cm bands and their actual migration size, the calibration coefficients of each bands and the corrective migration distance were calculated. This motion correction method can reduce the spectral error by about 2/3.