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为探讨B-RAF基因特异的siRNA干扰对胃癌BGC823细胞的增殖和凋亡的影响,设计并合成B-RAF小分子干扰RNA(B-RAF-siRNA)和阴性对照siRNA,用TransMessenger介导转染胃癌BGC823细胞,RT-PCR分析检测胃癌BGC823细胞中B-RAF基因以及Bcl-2基因的表达;MTT检测胃癌BGC823细胞增殖情况;流式细胞仪检测细胞凋亡情况,并与对照组进行比较。TransMessenger能够有效介导B-RAF-siRNA和阴性对照siRNA转染胃癌BGC823细胞,TransMessenger介导的B-RAF-siRNA有效地抑制胃癌BGC823细胞B-RAF以及Bcl-2基因的表达,与对照组相比,抑制率达90.0%以上,最高达100%;同时明显抑制胃癌BGC823细胞增殖;促进胃癌BGC823细胞的凋亡(P<0.01)。B-RAF基因特异的siRNA干扰能有效地抑制胃癌BGC823细胞中B-RAF基因以及Bcl-2基因的表达,同时促进胃癌细胞凋亡和抑制胃癌细胞增殖。
To investigate the effect of B-RAF gene-specific siRNA on the proliferation and apoptosis of gastric cancer BGC823 cells, we designed and synthesized B-RAF small interfering RNA (siRNA) and negative control siRNA. TransMessenger mediated transfection The expression of B-RAF gene and Bcl-2 gene in gastric cancer cell line BGC823 was detected by RT-PCR. The proliferation of BGC823 cell line was detected by MTT assay. Cell apoptosis was detected by flow cytometry and compared with that of control group. TransMessenger can effectively transduce B-RAF-siRNA and negative control siRNA into BGC823 gastric cancer cells. TransMessenger-mediated B-RAF-siRNA effectively inhibits B-RAF and Bcl-2 gene expression in gastric cancer BGC823 cells. Compared with control group The inhibitory rate was over 90.0%, up to 100%. At the same time, it significantly inhibited the proliferation of gastric cancer BGC823 cells and promoted the apoptosis of gastric cancer BGC823 cells (P <0.01). B-RAF gene-specific siRNA interference can effectively inhibit the expression of B-RAF gene and Bcl-2 gene in gastric cancer BGC823 cells, promote gastric cancer cell apoptosis and inhibit gastric cancer cell proliferation.