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为了对庚型肝炎病毒(HGV)的进一步研究提供基础,设计了针对HGV基因组5’-NCR区的引物,建立了RT-PCR检测HGV核酸的方法。通过对116例肝炎患者血清中HGV-RNA检测,分别在乙肝、丙肝患者血清中各检出2例HGV-RNA阳性,阳性率分别为4.7%和8.7%。甲肝患者血清中未检出HGV-RNA。
In order to provide a basis for the further study of hepatitis G virus (HGV), primers designed for the 5’-NCR region of HGV genome were designed and a method for detecting HGV nucleic acid by RT-PCR was established. HGV-RNA was detected in serum of 116 patients with hepatitis B, and 2 cases of HGV-RNA were detected respectively in serum of hepatitis B and hepatitis C patients. The positive rates of HGV-RNA were 4.7% and 8.7% respectively. HGV-RNA was not detected in the serum of hepatitis A patients.