论文部分内容阅读
目的 探讨食管癌及贲门癌组织中多药耐药相关蛋白(MRP)基因的表达与其病理特征的关系。方法 应用逆转录酶-多聚酶链反应(RT-PCR)方法,检测了83例食管癌和贲门癌组织及28例患者45枚淋巴结中MRP基因的表达,并与相应癌旁组织进行对照分析。结果 35例食管癌和48例贲门癌组织中MRP阳性率(31.4％和16.7％)与其对应癌旁组织阳性率(5.7％和2％)比较具有显著性差异(P<0.01)。26枚转移淋巴结中,MRP阳性6例(23.1％);19枚非转移淋巴结组织未见MRP。在食管癌和贲门癌侵犯深肌层或纤维层及TNM Ⅲ,Ⅳ期的MRP阳性率(26.6％和28.8％)高于肿瘤侵犯浅肌层以下和TNM Ⅰ,Ⅱ期(10.5％和12.9％),均分布于低分化肿瘤中。对48例术后1～2年的患者随访发现,MRP阳性的肿瘤复发、转移的发生率高于MRP阴性者(P<0.05)。结论 食管、贲门癌组织中MRP基因表达增高,与转移淋巴结组织的MRP基因表达具有一致性,MRP的阳性表达除表现肿瘤的多药耐药外,还提示肿瘤预后不良。
Objective To investigate the relationship between multidrug resistance associated protein (MRP) gene expression and pathological features in esophageal and gastric cardia cancerous tissues. The method of application of reverse transcriptase - polymerase chain reaction (RT-PCR) method, detection of the expression of the MRP gene in lymph nodes 45 and 83 cases of esophageal and gastric cardia 28 patients, with and adjacent tissues were compared. Results The positive rates of MRP (31.4% and 16.7%) in 35 esophageal and 48 cardia cancer tissues were significantly different from those in corresponding paracancerous tissues (5.7% and 2%) (P <0.01). Of 26 metastatic lymph nodes, MRP was positive in 6 cases (23.1%); no MRP was found in 19 non-metastatic lymph nodes. The positive rates of MRP (26.6% and 28.8%) in deep muscularis or fibrous layers and TNM Ⅲ and Ⅳ in esophageal and gastric cardia cancers were higher than those in superficial tumors and TNM Ⅰ and Ⅱ (10.5% and 12.9% ), Are distributed in poorly differentiated tumors. The follow-up of 48 patients 1-2 years after operation showed that the incidence of MRP-positive tumor recurrence and metastasis was higher than that of MRP-negative patients (P <0.05). Conclusions The expression of MRP gene in esophageal and gastric cardia cancer tissues is increased, which is consistent with MRP gene expression in metastatic lymph node tissues. The positive expression of MRP in addition to the multidrug resistance of tumor shows the poor prognosis of the tumor.